Part:BBa_K1648049:Experience

Double digestion of K1648049: L: DNA ladder. Lane 1-2: Recombination Template for R0040-oprF* (BBa_K1648049) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site.

Applications of BBa_K1648049

The ongoing experiment will be carried out for the comparison between the transformed bacteria with BBa_K1648048 for oprF from Azotobacter vinelandii BBa_K1648049 for mutated oprF from Azotobacter vinelandii and BBa_K1648050 for BBa_K1172501 from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.

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